一、个人简历

丁学峰，男，1990年09月出生，博士、博士后。

主要经历

2011.09—2015.06烟台大学，生命科学学院，学士

2016.09—2023.06江南大学，生物工程学院，博士

2023.07—至今江南大学，生命科学与健康工程学院，博士后

二、研究领域

（1）CHO细胞和293细胞稳定表达位点的寻找及鉴定

（2）治疗性药用蛋白的绿色生物制造

（3）CHO细胞和293细胞工程化改造

三、学术成果

（一）近5年来代表性论文

1、Yongqiang Deng,Xuefeng Ding（共一）, Qiyue Wu, Yanfei Cai, Jian Jin, Qinghe Xu, Jingyu Zhu, Yun Chen, (2023). Construction of a stable expression HEK293T engineered cell line adapted to high-density suspension culture,Biochemical Engineering Journal，Volume 207,2024,109334,ISSN 1369-703X.

2、Ding X,Chen Y, Yang Z, Wu H, Cai Y, Wang E, Jin J, Li H. (2023). A comprehensive evaluation of stable expression "hot spot" in the ScltI gene of Chinese hamster ovary cells.Appl Microbiol Biotechnol.107(4):1299-1309. doi: 10.1007/s00253-023-12383-w.

3、Hao Wu,Xuefeng Ding, Yun Chen, Yanfei Cai, Zhaoqi Yang, Jian Jin, (2023). EGFR-targeted humanized single chain antibody fragment functionalized silica nanoparticles for precision therapy of cancer,International Journal of Biological Macromolecules, Volume 253, Part 8,2023,127538, ISSN 0141-8130,

4、Hao Wu,Xuefeng Ding, Yun Chen, Yanfei Cai, Zhaoqi Yang, and Jian Jin. (2023). Constructed Tumor-Targeted and MMP-2 Biocleavable Antibody Conjugated Silica Nanoparticles for Efficient Cancer Therapy.nACS Omega, 8 (14), 12752-12760 DOI: 10.1021/acsomega.2c07949

5、Hao Wu, Xuefeng Ding, Yun Chen, Yanfei Cai, Zhaoqi Yang, Qinghe Xu, Jian Jin, (2023). Analysis of the in vitro function and internalization ability of a humanized EGFR antibody AE01 expressed by Chinese hamster ovary cells,Protein Expression and Purification, Volume 206, 106243, ISSN 1046-5928,https://doi.org/10.1016/j.pep.2023.106243.

6、Ding, XF;Chen, Y;Wu, H;Yang, ZQ;Cai, YF;Dai, YF;Xu, QH;Jin, J;Li, HZ,(2022).Construction of a CHO cell line with site-specific integration to stably express exogenous proteins using the CRISPR-Cas9 technique,SYSTEMS MICROBIOLOGY AND BIOMANUFACTURING,Volume3, Issue4,Page659-668.

7、Zhou, S.,Ding, X., Yang, L., Chen, Y., Gong, X., Jin, J., & Li, H. (2019). Discovery of a stable expression hot spot in the genome of Chinese hamster ovary cells using lentivirus-based random integration.Biotechnology & Biotechnological Equipment, 33(1), 605–612.https://doi.org/10.1080/13102818.2019.1601998

（二）近5年主要授权专利

1、2022.07，发明专利，一种CHO细胞基因NW_003614092.1内稳定表达蛋白质的位点及其应用，专利号：ZL202111395539.8

2、2022.07，发明专利，一种CHO细胞基因NW_003613781.1内稳定表达蛋白质的位点及其应用，专利号：ZL202111415460.7

3、2022.07，发明专利，一种CHO细胞基因NW_003614889.1内稳定表达蛋白质的位点及其应用，专利号：ZL202111395511.4

4、2022.07，发明专利，一种CHO细胞基因NW_003613756.1内稳定表达蛋白质的位点及其应用，专利号：ZL202111395490.6

四、联系方式

通讯地址：江苏省无锡市蠡湖大道1800号江南大学生命科学与健康工程学院

邮编：214122

邮箱：8202307003@jiangnan.edu.cn